HPLC法同时测定火锅添加剂产品中10种添加剂

Simultaneous determination of 10 kinds of food additives in Chafing dish additives by high performance liquid chromatography(HPLC)

目的:建立HPLC法同时测定火锅添加剂产品中防腐剂、甜味剂、人工合成色素等10种添加剂的检测方法。方法:采用超声溶剂浸提和固相萃取方法,提取待测物质,以甲醇/乙酸铵/水三元体系为溶剂,用C18反相柱分离被测物质,二极管阵列检测器测定10种添加剂。结果:采用超声溶剂浸提,各物质回收率可达80%以上;固相萃取的提取效率可达90%;三元溶剂体系可有效分离火锅添加剂中10种添加剂。方法线性好,相关系数r>0.999,相对准偏差可控制在5%以内。结论:本方法定量准确、重现性好,可同时测定火锅添加剂产品中的防腐剂、甜味剂和人工合成色素等10种添加剂。

Objective： To establish a method for simultaneous determination of 10 kinds of food additives, inclu- ding preservatives, sweeting agents and artificial color, in Chafing dish additives by HPLC. Methods： Preservative, sweeting agent and artificial color in Chafing dish additives were extracted by ultrasonic solvent and separated by C18 reversed phase column. The mobile phase is methanol/ammonium acetate/water and the target analytes were deter- mined by diode array detector （DAD）. Results： The recoveries of sample is more than 80% by ultrasonic solvent extraction, and up to 90% by solid phase extraction （SPE）. The mobile phase （ CH3OH - NH4Ac - H20） can sep- arate the 10 kinds of additives in sample. The correlation coefficient of regression equations（r） can be up to 0. 999 and relative standard deviation is within 5%. Conclusion： The method is simple and accurate, which can be appli- cable to determine preservative, sweeting agent and artificial color in Chafing dish additives.