MCX小柱SPE-HPLC法测定午餐肉中盐酸克伦特罗

Determination of clenbuterol hydrochloride in luncheon meat by SPE-HPLC with MCX column

目的：建立午餐肉中盐酸克伦特罗的SPE—HPLC检测方法。方法：用0．2moL／L盐酸溶液超声提取样品中的盐酸克伦特罗，加入亚铁氰化钾和乙酸锌沉淀蛋白质、淀粉和脂肪等，MCX固相萃取小柱净化、富集，以EclipseXDB—C18（4．6mm×150mm×8μm）为色谱柱，0．01mol／L磷酸二氢钠溶液（pH=6）：甲醇=65：35为流动相分离，流速为1．0ml／min，用DAD检测，检测波长为210nm。结果：在该色谱条件下，4．4min可达到基线分离，RSD为0．17％～0．24％，回收率为89．40％～97．62％。结论：该方法简便、准确，可用于肉类食品安全与质量监控。

Objective ： To establish a method for determination of clenbuterol hydrochloride in luncheon meat by SPE - HPLC.＂ Methods： The clenbuterol hydrochloride was extracted from the luncheon meat sample with 0.2 mol/L hydrochloric acid solution by ultrasonic extraction. Then potassium ferrocyanide and zinc acetate were added to precipitate the protein, starch and fat. Then MCX SPE column was used to purify and enrich the target analytes. The clenbuterol hydrochloride was separated on a Eclipse XDB - Cl8 （4.6 mm × 150 mm × 8 μm） chromato- graphic column with 0.01 mol/L NaH2PO4 （pH = 6） ： methanol = 65：35 as mobile phase at a flow rate of 1.0 mL/min, and detected with the DAD at wavelength of 210 nm. Results： Under this chromatographic condition, baseline was separated in 4.4 min, the RSD was 0.17% - 0.24%, and the recoveries were 89.40% - 97.62%. Conclusion： The method is simple, accurate, and it can be used to control meat product quality and security.