薄荷素油的气相色谱法指纹图谱研究

Study on fingerprint chromatogram of peppermint oil by GC

目的:建立薄荷素油的GC指纹图谱测定法,为薄荷素油质量控制提供参考。方法:采用HP-FFAP弹性石英毛细管柱,火焰离子化检测器(FID),测定了不同生产厂家的薄荷素油的GC指纹图谱,通过中药色谱指纹图谱相似度评价确定了其对照指纹图谱,并对方法进行了一系列的验证试验,通过GC-MS确定了薄荷素油的主要化学成分。结果:建立的薄荷素油GC指纹图谱测定方法分离良好、专属性强,可用于薄荷素油质量的比较和控制。结论:以特征对照品为参照进行薄荷素油指纹图谱相似度评价,结果可靠,重复性好,可全面有效地控制薄荷素油的质量。

Objective： To establish the stable and repeatable GC fingerprint chromatograph standard for quality analysis and control of peppermint oil. Methods： GC separation was performed on HP - FFAP column with FID as detector. Reference fingerprint chromatograph was established by the software of chromatographic fingerprint similari- ty evaluation. The method was verified by a series of tests. The main chemical ingredients were determined by GC - MS. Results： The results indicated that the quality assessments of peppermint oil with reference fingerprint chroma- tograph had good ruggedness, specificity and reproducibility. Conclusion： The GC fingerprint chromatograph as- sessment method with characteristic standards reference is simple, accurate and reproducible. It can provide a relia- ble way for evaluating the quality of peppermint oil.