甘肃人群人类血小板抗原HPA-15多态性调查

Investigation on gene frequency of human platelet antigen-15 system in Gansu

目的:研究甘肃人群人类血小板抗原HPA-15系统多态性,评估其在血小板配型输注中的作用。方法:采用PCR-SSP技术对201名健康无血缘关系的血小板捐献者进行HPA-15系统基因分型。结果:HPA-15a和15b基因频率分别为0.6194和0.3806;同国内其它9个地区人群的HPA-15分布相比较,与7个地区人群有显著性差异。在随机输血中HPA-15a和15b抗原不配合的机会分别为0.1239和0.2365,a、b抗原不配合率合计为0.3604。结论:本调查数据表明,随机输血中供受者HPA-15抗原不配合比例在甘肃人群中高达36.04%,这对研究血小板同种免疫性疾病和开展相合血小板输注具有指导意义。

Objective： To study the polymorphism of human platelet antigen HPA - 15 system in Gansu popula- tion, and evaluate its role in matching platelet transfusion. Methods： The blood samples were collected from 201 healthy unrelated platelet donors in Gansu blood center for HPA - 15 system genotyping using PCR - SSP assay. Re- suits： Gene frequencies of HPA - 15a and 15b were 0. 6194 and 0. 3806. In comparison to other 9 ethnic groups, there were 7 ethnic groups showing significant differences in HPA - 15 distribution. The incompatibility ratios in random platelet transfusion were 0. 1239 and 0. 2365 for HPA - 15a and - 15b antigens, total of 0. 3604. Conclu- sion： The distribution of HPA - 15 system in Gansu population were investigated for the first time. The present data indicate that the ratio of HPA - 15 antigen incompatibility in random platelet transfusion is up to 36.04%, providing a basis for further study on matched platelet transfusion.