HPLC法测定甘草制剂中甘草酸的含量

HPLC method for content determination of glycyrrhizic acid in Radix Glycyrrhizae preparations

目的:建立HPLC法测定不同甘草制剂中甘草酸的含量。方法:采用Hypersil BDS C18(4.6mm×250mm,5μm)色谱柱,流动相为10mmol/L醋酸铵溶液(pH=6.86)-乙腈(79.5∶20.5),流速1.0mL/min,柱温38℃,检测波长250nm。结果:甘草酸在0.26～41.6μg/mL范围内线性关系良好(r2=0.9998)。各甘草制剂中甘草酸均得到良好的分离,且含量各不相同,复方水煎液为2.17μg/mL,复方甘草口服溶液为3.07mg/mL,复方甘草片为5.88%,甘草提取物为10.75%。结论:该方法准确,简便,重复性好,适用于各种甘草制剂中甘草酸含量的测定。

Objective: To establish a method for determining the content of glycyrrhizic acid in different Radix Glycyrrhizae preparations by high-performance liquid chromatography(HPLC).Methods: HPLC was performed on a Hypersil BDS C18 column(4.6 mm × 250 mm,5 μm) with a mobile phase of 10 mmol / L ammonium acetate solution(pH = 6.86)-acetonitrile(79.5: 20.5) at a flow rate of 1.0 ml / min,a column temperature of 38℃,and a detection wavelength of 250 nm.Results: Glycyrrhizic acid had a good linear relationship between concentration and peak area in the concentration range of 0.26-41.6 μg / ml(r2 = 0.9998).Glycyrrhizic acid was well separated from each Radix Glycyrrhizae preparation.The content of glycyrrhizic acid was 2.17 μg / ml in compound water decoction of Radix Glycyrrhizae,3.07 mg / ml in compound oral liquid of Radix Glycyrrhizae,5.88% in compound Radix Glycyrrhizae tablets,and 10.75% in Radix Glycyrrhizae extract.Conclusion: This HPLC method is accurate,simple,and highly repeatable,and can be used for content determination of glycyrrhizic acid in various Radix Glycyrrhizae preparations.