树鼩对丙型肝炎病毒的易感性研究

INVESTIGATION OF SUSCEPTIBILITY OF TREE SHREWS TO HEPATITIS C VIRUS

目的 :探讨树对丙型肝炎病毒 (HCV)的易感性。方法 :对来自云南的树接种含 HCV的病人血清 ,然后用 PCR方法检测树血清的 HCV RNA,用反向被动血凝法测定抗 -HCV抗体 ,用原位 PCR方法检测树肝细胞内的 HCV RNA。结果 :34 .8%的树 (8/ 2 3)在接种 HCV后 ,血清中检出 HCV RNA,对 4例抗 HCV阳性和谷丙转氨酶升高的树的肝组织进行原位 PCR检测 ,结果在 1例肝细胞中检出 HCV RNA。此外 ,30 .4 %的树 (7/ 2 3)在接种后出现抗 -HCV抗体。部分阳性树均有不同程度的谷丙转氨酶异常升高及肝组织病理性炎症反应。对照组动物 (2只 )从未出现 HCV RNA及抗 -HCV抗体 ,但其中一只树曾有一过性转氨酶升高。结论 :树可以感染 HCV。

Objective:To study the susceptibility of tree shrews to hepatitis c virus (HCV)Methods:The blood of tree shrews obtained from Yunan China and inoculated with HCV was examined for the presence of HCV RNA by PCR and antiHCV antibody by passive hemagglutination assayIn addition,liver tissue obtained from serum HCV RNA positive tree sherw was analyzed by in situ PCRResults:348% of tree shrews(8/23) developed viremia and 304% (7/23) presented antiHCV antibody after inoculationHCV RNA was also found in the hepatocytes of a tree shrew whose serum was positive for HCV RNAHowever,neither HCV RNA nor antiHCV was found in control tree shrewsAlanine aminotransferase (ALT) was elevated in some infected tree shrews and one control animalConclusion:Tree shrew is susceptible to HCV