ANXA7基因mRNA水平在神经胶质瘤中的研究

Significance of ANXA7 gene mRNA levels in glioblastoma

目的研究ANXA7基因mRNA水平与神经胶质瘤的相关性。方法选择神经胶质瘤患者70例,健康对照组24例。提取脑组织mRNA,设计定量PCR引物与探针,经PCR扩增进行荧光定量分析。结果定量PCR检测结果显示,24例正常脑组织ANXA7基因mRNA水平高于70例神经胶质瘤患者(p=0.001);同时高级别神经胶质瘤患者ANXA7基因mRNA水平低于低级别组(P=0.01)。最后我们采用DNA甲基化特异性PCR技术(MS-PCR)检测70例神经胶质瘤患者ANXA7启动子区甲基化水平,并与其mRNA水平进行相关性分析,结果显示高甲基化组ANXA7基因mRNA水平显著低于低甲基化组(p=0.003)。结论 ANXA7基因表达降低与ANXA7基因启动子区甲基化水平有一定相关性,与神经胶质瘤的发生发展密切相关,为疾病进展的早期监测提供分子理论依据。

Objective The aim of this study was to evaluate the significance of ANXA7 gene mRNA levels in Glioblastoma.Methods Seventy patients with Glioblastoma and 24 healthy donors were adopted in this study.RNA was isolated from cerebra tissue,and quantitative real-time PCR（Q-PCR） were used to detect the levels of ANXA7 gene in health donors and newly diagnosed Glioblastoma patients.Results The results indicated that Q-PCR analysis showed the levels of ANXA7 mRNA in the group of Giloblastoma were lower than that in the group of healthy donors（p = 0.001）.Furthermore,the ANXA7 expression was lower in high stage disease than that in the group of low stage（p = 0.01）.Lastly,we used MS-PCR to detect the methylation levels of the ANXA7,and found that the levels of ANXA7 mRNA in the group of high methylation were lower than that in the group of low methylation（p =0.003）.Conclusion The aberrant expression of the ANXA7 gene is perhaps correlated with the levels of ANXA7 methylation and may be involved in the occurrence and provides of Glioblastoma,and provideds the molecular basis of the early monitoring disease.