卵巢早衰小鼠中抗苗勒管激素与卵泡刺激素受体相关性的研究

Relationship between anti-Miillerian hormone level and expression of follicle-stimulating hormone receptor in premature ovarian failure mice

目的探讨卵巢早衰(POF)小鼠中抗苗勒管激素(AMH)与卵泡刺激素受体(FSHR)的关系。方法以小鼠透明带3(ZP3)的第330～342个氨基酸序列所合成的透明带多肽为免疫原,免疫SPF级Balb/c雌性小鼠,皮下多点注射建立POF模型。放射免疫法测定模型组及对照组小鼠外周血FSH、雌二醇(E_2)的水平,酶联免疫法测定外周血AMH、抗透明带抗体(AzpAb)的水平,HE染色观察卵巢组织的变化,并用免疫组化方法分析卵巢颗粒细胞中FSHR的表达。结果 POF小鼠较正常小鼠外周血的FSH升高,E_2值降低,AMH值降低,AzpAb值升高。POF小鼠卵巢的HE染色病理切片见卵泡闭锁,正常发育的卵泡甚少。免疫组化分析,FSHR仅在颗粒细胞上表达,且POF小鼠卵巢上FSHR的表达明显少于正常组。结论 FSHR仅在颗粒细胞上表达,POF小鼠中各级卵泡的FSHR表达均降低,POF小鼠伴低浓度AMH,且FSHR的表达随AMH降低亦降低。

Objective： To study the relationship between anti-Miillerian hormone （AMH） level and expression of follicle-stimulating hormone receptor （FSHR） in mice with premature ovarian failure. Methods. The peptide consisting of the first 330-342 amino acid sequences of mouse zona pellucida 3 was synthesized and used as immunogen to immunize Balb/c female mice of specific pathogen free （SPF） grade with subcutaneous injection to establish the model of premature ovarian failure （POF）. FSH and E2 levels in serum were measured by radioimmunoassay. The AMH and anti-zona pellucida antibody （AzpAb） levels in the serum were measured by enzyme-linked immunosorbent assay （ELISA）. The ovarian tissue was stained with HE. The FSHR expression in the ovarian granulosa cells was analyzed by immunohistochemistry. Results： Increased FSH and AzpAb levels, decreased E2 and AMH levels and follicular atresia were found in POF mice. A few normal development follicles were observed in POF mice. The expression of FSHR was only seen in the granulosa cells of follicle and the FSHR expression was less in POF mice. Conclusions. In POF model mice, the expression of FSHR is only in the granulosa cells of follicle, and its expression being lower. The AMH concentration also is lower, and in the AMH low concentration range, along with AMH concentration decreasing, FSHR expression was also reduced in granulosa cells.