冻融成人卵巢组织异种移植后形态和功能的研究

A study on the morphology and function of adult ovarian tissues after cryopreservation and xenotransplantation

目的探讨经玻璃化法冷冻保存的成人卵巢组织异种皮下移植后卵泡生长发育和雌激素分泌情况。方法将30只SCID小鼠随机分为2组:去势对照组和移植组。两组小鼠均于术后7 d开始隔日给予重组卵泡刺激素(rFSH)及重组黄体生成素(rLH)各5 U。移植术20周后将获取的移植物进行光学显微镜观察,并测定两组小鼠血清雌二醇(E_2)浓度及子宫相对重量。结果卵巢皮质移植物内可以观察到各级卵泡发育,移植组小鼠血清E_2水平及子宫相对重量高于去势对照组,差异有统计学意义(P<0.05)。结论经玻璃化冷冻保存并异种皮下移植的人类卵巢组织可以存活,具有雌激素分泌功能,并作用于靶器官。

Objective： To explore the restoration of ovarian estradiol （E2） secretion and the development of follicles after subcutaneous xenotransplantation of human ovarian tissue cryopreserved by vitrification. Methods. Thirty SCID mice were divided into 2 groups, ovariectomized control group and xenografted ovarian group. Seven days later, each mouse was injected with 5 U of rFSH and 5 U of rLH every two days for 20 weeks. On 20 weeks after operation, the grafted ovarian tissues were obtained and estimated under microscopy. The weight of uterus and the level of E2 were measured. Results： The proportions of growing follicles were found in the survival grafts. The weight of uterus and the E2 levels in the xenografted ovarian group were significantly higher than those in the ovariectomized control group （P〈0.05）. Conclusions. The vitrification frozen-thawed adult ovarian tissues can survive in mouse. The follicles could develop and E2 well secrete in xenografted ovarian transplanted into the mouse subcutaneous tissue.