摘要
目的应用基于相位衬度成像技术的显微CT(Micro-CT)对小鼠听泡进行形态学观察,建立中耳、内耳的3D模型,探讨Micro-CT在耳科影像学上的应用。方法颈椎脱臼处死小鼠,剥离听泡,苦味酸固定后行Micro-CT扫描,获得2D图像,应用Amira-5.4建模重构获得3D模型。结果获得了空间视觉良好的3D模型,鼓膜、中耳听骨链及其局部结构清晰,内耳前庭、半规管、耳蜗形态完整,内部微结构显像;听泡模型整体立体透视良好,可做任意的轴向旋转,得到所需目标图像。结论应用Micro-CT对小鼠听泡进行无损成像,可同时获得标本的外部形貌和内部细节特征,所获图像分辨率高,建模重构所获3D模型空间视觉较好。Micro-CT样本制备过程简单,操作便捷,设备占用空间小,具有良好的发展前景。
Objective To get the three-Dimensional imaging model of internal ear and middle ear using the Micro-CT equipment, and research the nondestructive imaging of mice acoustic capsule, and investigate the application of Micro-CT inmorphology of acoustic capsule. Methods The mice were executed to harvest the acoustic capsules. The acoustic capsules were fixed in trinitrophenol before scanning by Micro-CT . Using this equipment, we obtained a 3D model of reconstruction from 2D images. Results The 3D model of acoustic capsule and the model was extraordinary clear to show the eardrum, ossicles chain, vesfibulum, semicircular canal, and cochlear. The 3D structure model was excellent for look through, and it was free to revolve in any axial direction on the platform of software Amira-5.4. Conclusions By using Micro-CT equipment to research the nondestructive three-Dimensional imaging of acoustic capsule, the outside structures and internal details of the acoustic capsule can be observed at the same time. 3D structure model which was good for looking through. Using Micro-CT equipment is easy to operate and also can save a lot of samples, and the images have high resolution ratio. Micro-CT has good prospects for development.
出处
《中华耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2013年第8期673-676,共4页
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
基金
国家自然科学基金青年基金(NSFC-11205189)
国家外专局专项基金
关键词
X线显微体层摄影术
耳
成像
三维
小鼠
X-ray mierotomography
Ear
Imaging, three-dimensional
Miee