肥大细胞Toll样受体4在人慢性牙周炎牙龈组织中的表达

Toll-like receptor 4 expression on mast cells in human gingival tissues with chronic periodontitis

目的:观察人慢性牙周炎牙龈组织中肥大细胞Toll样受体4(Toll-like receptor 4,TLR4)的表达,并探讨其在牙周炎发病中的可能作用。方法:将68例自愿接受本研究的牙周炎患者按照牙周炎的病变程度分成3组:轻度牙周炎组(23例)、重度牙周炎组(25例)和正常对照组(20例)。取牙龈组织标本,4%中性甲醛液固定48 h以上。制作牙龈组织的连续切片,HE染色,光学显微镜下观察各实验组牙龈组织的组织学改变;免疫组化法染色观察各实验组牙龈组织TLR4的表达;免疫荧光双染色法观察TLR4在肥大细胞中的表达。结果:(1)与正常对照组相比,各慢性牙周炎组牙龈组织中TLR4的表达及TLR4在肥大细胞中的表达均显著升高(P<0.05);(2)重度牙周炎组牙龈组织中TLR4表达的数量及TLR4在肥大细胞中的表达明显高于轻度牙周炎组(P<0.05)。结论:TLR4在牙龈组织中的表达及在肥大细胞中的表达量随着牙周炎的炎症程度加重而增加,提示TLR4,特别是肥大细胞TLR4可能在慢性牙周炎的发病和疾病进程中起着重要的作用。

AIM： To observe the expression of Toll-like receptor 4 （TLR4） on mast cells in human gingival tissues with chronic periodontitis. METHODS ： A total of 68 volunteers, including 23 cases of mild chronic periodontitis, 25 cases of severe chronic periodontitis and 20 healthy controls, were involved in this study, and their gingival specimens were taken and fixed in 4% neutral formalin. The histological changes of gingival tissues were observed by HE staining. The expression of TLR4 in gingival tissues was detected by immunohistochemical staining, and TLR4 expression on mast cells was detected by immunofluorescence double staining. RESULTS： The expression of TLR4 in gingival tissues and on mast ceils in chronic periodontitis groups was significantly higher than that in normal control group （ P 〈 0. 05 ）, and that in severe chronic periodontitis group was significantly higher than that in mild chronic periodontitis group （P 〈0. 05）. CONCLUSION： The expression of TLR4 in gingival tissues and on mast ceils is increased with the severity of chronic periodontitis, suggesting that TLR4, especially TLR4 on mast cells, may play an important role in human chronic periodontitis. [