红花注射液对脂多糖诱导的兔弥散性血管内凝血的作用

Effect of safflower injection on LPS-induced disseminated intravascular coagulation in rabbits

目的:研究红花注射液对脂多糖(LPS)诱导的兔弥散性血管内凝血(DIC)的作用。方法:兔耳缘静脉持续滴注LPS建立兔DIC模型。采用全自动凝血分析仪检测活化部分凝血活酶时间(APTT)和凝血酶原时间(PT);凝固法测定纤维蛋白原含量;全自动血细胞分析仪进行血小板计数;发色底物法测定蛋白C及抗凝血酶Ⅲ的活性;全自动血浆分析仪测定丙氨酸氨基转移酶(ALT)和血尿素氮(BUN);ELISA法检测血浆纤维蛋白(原)降解产物(FDP)和肿瘤坏死因子α(TNF-α)含量。结果:DIC模型组APTT和PT进行性延长,ALT活性和BUN含量显著升高,蛋白C和抗凝血酶Ⅲ的活性显著降低,血小板计数进行性减少,血浆FDP和TNF-α含量显著升高。给予红花注射液后,DIC模型兔APTT和PT的延长明显缩短,血小板计数、纤维蛋白原含量、蛋白C及抗凝血酶Ⅲ活性均明显恢复,血浆ALT、BUN、FDP和TNF-α水平均明显降低。结论:红花注射液对LPS诱导的兔DIC有较好的拮抗作用。

AIM： To study the effect of safflower injection on lipopolysaccharide （LPS）-induced disseminated intravascular coagulation （DIC） in rabbits. METHODS： The rabbit model of DIC was induced by continuous infusion of LPS at a dose of 100μg·kg^-1· h^-1 for 6 h. The treatment with safflower injection and heparin were started simultaneous- ly with LPS infusion through the contralateral marginal ear vein. The activated partial thromboplastin time （APTY）, prothrombin time （PT）, platelet count and fibrin（ogen） concentration were measured. The plasma levels of fibrin（ogen） degradation products （FDP）, alanine aminotransferase （ALT） and blood urine nitrogen （BUN） were detected. The activity of protein C and antithrombin III（ AT III） and the concentration of tumor necrosis factor α （TNF-α） were also determined. RESULTS： The gradual injury of hemostatic parameters was induced by continuous infusion of LPS. APTT, PT, BUN, ALT and plasma TNF-α were increased obviously. The platelet count, fibrinogen, FDP, protein C and AT III were decreased significantly. The intravenous administration of safflower injection attenuated the increases in the levels of APTT, PT, BUN, ALT, FDP and plasma TNF-α induced by LPS infusion, and improved the plasma levels of fibrinogen, platelet, protein C and AT III. CONCLUSION： Safflower injection protects against LPS-induced DIC in rabbits. [