香砂六君子汤对脾气虚证大鼠肠神经-ICC间信号转导通路损伤的作用

Expression Changes of Signal Transduction Pathway of Enteric Nerves-Interstitial Cells of Cajal in Rat with Deficiency of Spleen-Qi Syndrome and Therapeutic Effects of Xiang Sha Liu Jun Zi (香砂六君子) Decoction

目的:观察脾气虚证大鼠肠神经Cajal间质细胞(ICC)间信号转导通路的表达改变和香砂六君子汤的治疗作用。方法:将100只Wistar大鼠随机分为对照组、模型组、自然恢复组和香砂六君子汤治疗组,采用免疫荧光双标记染色、激光扫描共聚焦显微镜观察模型组大鼠小肠肠神经-ICC信号转导通路损伤和香砂六君子汤的修复作用。结果:脾气虚证大鼠小肠ICC计数和荧光强度(IOD)值明显降低,网络样结构遭到破坏,PKA/PKC蛋白荧光强度IOD值明显降低。结论:香砂六君子汤能够修复脾气虚证大鼠小肠Cajal间质细胞和信号转导通路的损伤,改善胃肠运动障碍,治疗脾气虚证。

Objective To observe the expression changes of signal transduction pathway of enteric nerne system-interstitial cell （ENS-ICC） and the therapeutic effects of Xiang Sha Liu Jun Zi decoction（XSLJZD） in rats with deficiency of Spleen-Qi syndreme （DSQS）.Methods One hundred Wistar rats were randomly divided into control group,DSQS group,recovery group and XSLJZD group.The signal transduction pathway of ENS-ICC of the gastrointestinal tract was observed by using immunohistochemical double-staining with whole-amount preparation technique and confocal laser scanning microcopy.Results The IOD of ICC of intestine in DSQS group was significantly lower than that in control group（P0.01）,the ICC network was significantly disrupted.The IOD of ICC of intestine in the recovery group was slightly better than that in DSQS group（P 0.01）,the ICC network was slightly recovered.The IOD of ICC of intestine in XSLJZD treatment group was significantly increased（P 0.01）.After treatment with XSLJZD,the ICC network was significantly recovered.The IOD of protein kinase A（PKA）/protein kinase C（PKC） in DSQS group was significantly decreased（P0.01）.The signal transduction pathway was destroyed.The IOD of protein kinase A（PKA）/protein kinase C（PKC） in the recovery group was slightly recovered（P 0.01）.While the IOD of protein kinase A（PKA）/protein kinase C（PKC） in XSLJZD treatment group was significantly increased.The signal transduction pathway was significantly recovered.Conclusion XSLJZD can improve the gastrointestinal motility in DSQS and the mechanism may be related to the repairing effect on the distribution and the connection of Signal Transduction Pathway of ENS-ICC in rat with DSQS.