正交试验结合药效学实验优选复方南星止痛膏处方提取工艺

Optimization of extraction process of prescription of compound Nanxing pain-relieving cataplasm by combining orthogonal design and pharmacodynamic experiment

目的:通过正交试验及镇痛抗炎实验探讨复方南星止痛膏处方最佳提取工艺。方法:采用正交试验法优选白芷、川芎等药材醇提工艺;采用鼠尾压痛及小鼠足趾肿胀药效学实验对丁香等药材中挥发油不同提取方法的提取物进行镇痛及抗炎作用对比,优选丁香等药材中挥发油的提取工艺。结果:复方南星止痛膏处方白芷、川芎等药材最佳醇提工艺为加入8倍量70%乙醇,提取2次,每次1.5 h;丁香等药材95%醇提液对小鼠痛阈值的增大作用及小鼠足趾肿胀的抑制作用大于水蒸气蒸馏所得的挥发油及水煎液。结论:该法简便、可靠,可为复方南星止痛膏现代制剂的开发提供技术参考。

Objective： To discuss the optimum extraction process of compound Nanxing pain-relieving cataplasm through or- thogonal design and pharmacodynamic experiment. Method： The orthogonal experiment method was adopted to optimize the ethanol ex- traction process with Angelica dahurica, Ligusticum chuanxiong and other herbs. The anti-inflammatory and analgesic effects of extracts from volatile oil in such herbs as syzygium aromatieum with different extraction processes were compared by tail pain tenderness test and food-pad swelling in mice, in order to optimize the extraction process of extracts from volatile oil in such herbs as syzygium aromaticum. Result： The optimum extraction process of A. dahurica, L. chuanxiong and other herbs for compound Nanxing pain-relieving cataplasm were as follows ： adding 8-fold amount of 70% alcohol, extracting for 2 times with 1.5 h each time. The 95% ethnol extracts of syzygium aromaticum and other herbs had more effect in the increasing the threshold of pain and the inhibition of toe swelling of mice than volatile oil obtained from steam distillation as well as volatile oil and water decoction obtained from steam distillatiorL Conclusion： The method is simple and reliable that it can provide technical reference for the development of modern preparations of compound Nanxing pain-relieving cataplasm.