吴茱萸果实的肝脏毒性研究及毒性部位初步探索

Hepatic Toxicity Study on the Fruit of Evodia rutaecarpa ( Juss. ) Benth

目的对吴茱萸的肝脏毒性提供证据。方法①肝脏毒性研究中,将ICR小鼠平分为四组,其中三组作为剂量组分别经口灌胃给予吴茱萸水煎剂40,60,80 g/kg体重,最后一组给予蒸馏水。所有动物均在24 h以后处死,并且测量谷丙转氨酶,谷草转氨酶,乳酸脱氢酶和总胆红素。同时观察肝脏的病理组织学变化。②将吴茱萸水煎剂用氯仿,正丁醇直接萃取分为三个极性部分并且准备了挥发油。所有组均是分别经口以120 g/kg给予以上萃取物。24 h后测定ALT。结果比较对照组,60 g/kg,80 g/kg组的小鼠的血清ALT,AST,LDH和T-Bil活性均明显升高(P<0.01)。病理组织学研究也表明了肝脏组织的变化。氯仿提取物受试组小鼠血清ALT有明显升高(P<0.05)。结论吴茱萸水煎剂有肝脏毒性作用;毒性成分存在于氯仿萃取部分。

Objective This study aims to provide evidence for hepatic toxicity. Methods （ 1 ） Male ICR mice were divided into four groups, of which three groups were treated with the decoction of Fructus Evodiae in a dose of 40,60,80 g/kg body weight respectively,the last were treated with distilled water. After 24 h, ALT, AST, LDH and T - bil were measured. Histopathological changes of liver were observed. （ 2 ） We divided the decoction of Fruetus Evodiae by chloroform, n - butyl alcohol into three extraction parts and prepared volatile oil extract. All groups were orally treated with above extracts in a dose of 120 g/kg body weight once. ALT was measured after 24h. Results Serum ALT, AST, LDH and T - Bil activities of 60g/kg, 80g/kg groups significantly increased than the vehicle control group （P 〈 0.01 ）. Histopathology studies also indicated a change of liver. The serum of chloroform extract group presented a significant increasing activity in ALT （ P 〈 0.05 ）. Conclusion It demonstrates that the decoction of Fructus Evodiae had hepatic toxic effect. Until now,we found the toxic ingredients lied in its chloroform extract fraction.