狭叶松果菊的毛状根诱导及培养

Induction and in vitro Culture of Echinacea angustifolia Hairy Roots

目的利用两种发根农杆菌A4,R1000诱导狭叶松果菊产生毛状根,建立狭叶松果菊的毛状根培养体系。方法利用共培养法研究不同外植体、菌株、预培养时间和浸染时间等对狭叶松果菊毛状根诱导率的影响和不同液体培养基对毛状根生长的影响筛选出最佳培养基。结果利用发根农杆菌R1000,预培养48 h的叶柄为转化材料,浸染10 min的诱导率最高,毛状根悬浮培养的最佳培养基为1/2MS+IBA0.5液体培养基。结论狭叶松果菊毛状根离体培养体系的建立,为狭叶松果菊的次生代谢产物的大规模生产奠定了基础。

Objective To induce hairy roots of Echinacea angustifolia by two strains of Agrobacterium rhizogenes A4 and R1000 and establish an in vitro culture system of the hairy roots. Methods Hairy roots were induced by cocuhure Effects of explants, Agrobacterium rhizogenes, preculture time along with infecting time on the induction rate and effects of different basic media on growth of hairy root were studied. Results The highest induction rate was obtained from leaves with 48h preculture which were induced by R1000 for 10min. The growth of hairy root could be raised by 1/2MS medium with 0.5 mg/L IBA. Conclusion Establishment of Echchina angustifolia hairy root culture can provide a foundation for the industrial production of active drug component.