5/6肾切除大鼠慢性肾衰病理机制研究

Pathological Mechanism Research on 5/6 Nephrectomy in Rats with Chronic Renal Failure

目的:探究5/6肾切除所致慢性肾衰动物模型在病理发展过程中血中生化指标及肾脏基因表达的变化。方法:将SD大鼠随机分为假手术组(Sham)和5/6肾切组(NX),分别于术后6周、8周末处死,收集血清、血浆和肾脏组织,测定血清肌酐、血浆醛固酮含量及肾脏组织TGF-β1、TNF-α和IL-17基因的表达。结果:病理组动物血清肌酐、血浆醛固酮含量较假手术组显著性升高,肾脏组织TGF-β1、TNF-α和IL-17基因表达较假手术组升高,且8周病理组各项指标升高幅度大于6周病理组。结论:RAAS参与慢性肾衰病变过程,引发肥大、炎症等一系列变化,并且随时间延长而病程加深。

Objective：To explore the changes of biochemical indicators in blood and gene expression in kidney in the the pathological development process of 5/6 nephrectomized animal models.Methods：SD rats were randomly divided into sham operation group（Sham） and the 5/6 nephrectomy group（NX）,which were respectively sacrificed to collect serum,plasma and kidney tissue after 6 week and 8 week in order to to measure serum creatinine,plasma aldosterone content and kidney tissue TGF-β1,TNF-α and IL-17 gene expression.Results：In NX group,the serum creatinine,plasma aldosterone content and TGF-β1,TNF-α and IL-17 gene expression in kidney tissue significantly increased compared with sham group,and indicators of the NX group（8w） has greater high magnitude than NX group（6w）.Conclusion：RAAS is involved in the disease process of chronic renal failure,triggering a series of changes such as hypertrophy,inflammation,and the course of diease deepens along with the time.