HPLC法测人体精液中2,3-吲哚醌的含量

Determination of ISA in human semen by HPLC

目的建立测定人体精液中2,3-吲哚醌(ISA)浓度的液相方法。方法精液处理后用乙酸乙酯提取,提取液吹干加盐酸溶解后进样,采用高效液相色谱仪外标法检测2,3-吲哚醌的含量结果 2,3-吲哚醌在30～1000μg/L范围内线性关系良好(r=0.9996),回归方程为A=87.366c-381.68,最低检测限为9ng。结论该测定方法准确可靠,可用于精液中2,3-吲哚醌的含量测定。

Objective：To establish a method to determination ISA in human semen by HPLC.Methods：The semen was extract by ethyl acetate after processed,and the extract was blown dry by nitrogen.The content was determined by HPLC with External standard method.Results：The linear regression for ISA ranged from 200 to 5000μg/L（r=0.9998）,regression equation is A=86.834c-378.89.The limit of detection（LOD） is 9ng.Conclusion：This method was sensitive,accurate.And it can be used for the determination of ISA.