绵羊肺炎支原体贵州分离株的鉴定

Isolation and identification of Mycoplasma ovipneumoniae in Guizhou province

【目的】对贵州省疑似绵羊肺炎支原体(Mo)感染山羊病例进行病原确定性诊断。【方法】采集疑似Mo感染山羊肺脏,以支原体专用培养基从其中分离致病支原体,并通过形态学观察、生化试验、血清学试验及分子生物学方法对分离菌株进行鉴定。【结果】分离菌株菌落呈无中心脐圆形凸起,菌体呈多形性,大小在0.2～0.4μm;分离株表现出了与Mo标准株Y98相同的生化特性,在含抗Mo血清培养基中不生长;分离株16SrRNA基因序列与Mo Y98株同源性达99.55%,系统进化树分析与MoGH3-3株进化关系最近。【结论】成功分离到了Mo,为确定本地山羊支原体肺炎病原种类提供了理论依据,并为后续围绕病原进行防控工作研究奠定了基础。

【Objective】 This study accurately diagnosed the cause of goats suspiciously infected with Mycoplasma ovipneumoniae（Mo） in Guizhou province.【Method】 The pathogenic mycoplasma was isolated from lung of goat infection by specific culture medium and was identified through morphological observation,biochemical test,serological test and molecular biological assay.【Result】 The results showed that the colony of isolation presented circular projection without central umbilical cord and single was multiple morphological with size between 0.2 μm and 0.4 μm.The isolation behaved common biochemical characteristics with Mo standard strain Y98,and could not grow in culture medium containing serum against Mo.The sequence of 16S rRNA gene of the isolation was also obtained and analyzed by homology comparison and phylogenetic tree construction,which showed homology of isolation with Mo strain Y98 was 99.55% and phylogenetic relationship was the nearest to strain MoGH3-3.【Conclusion】 Comprehensive analysis proved the isolation was Mo and helped the research to determine local pathogenic strain and control Mo spreading continuously.