藏药川西獐牙菜抗小鼠免疫性肝损伤的研究

Resistance of Swertia mussotii Franch against immunological liver injury of mice

【目的】探讨川西獐牙菜对卡介苗(BCG)/脂多糖(LPS)所致的免疫性肝损伤小鼠免疫器官脏器指数和血清免疫球蛋白含量的影响。【方法】采用尾静脉注射BCG和LPS构建小鼠(体质量20g)免疫性肝损伤模型。制备川西獐牙菜提取物,按高剂量(5.380mg/只)、中剂量(2.690mg/只)、低剂量(0.538mg/只)灌胃免疫性肝损伤小鼠,连续10d后,取小鼠肝脏、脾脏和胸腺,计算其脏器指数,并取血清,测定其中IgG、IgA、IgM的含量。【结果】川西獐牙菜的各给药组能有效防止BCG/LPS对小鼠各脏器的免疫性损伤,并能影响IgG、IgA、IgM的含量,调节B淋巴细胞网络的紊乱,使异常的免疫状态恢复正常。【结论】川西獐牙菜预防性给药能减轻免疫性肝损伤对小鼠肝脏的破坏,减弱体液免疫功能的亢进,从而起到保肝、抗病、抗损伤的作用。

【Objective】 This study investigated the effects of Swertia mussotii Franch on the immune organ indexes and serum immunoglobulin content of the immunological liver injury in mice caused by BCG vaccine and lipopolysaccharide.【Method】 Mice with immunological liver injury were（mice body mass was 20 g） caused by intravenously injection of BCG vaccine and lipopolysaccharide.The extract of S.mussotii Franch was intragastric administrated to mice at three levels： high dose（5.380 mg/a mouse）,intermediate dose（2.690 mg/a mouse） and low dose（0.538 mg/a mouse）.After ten days of continuous fed,the liver,spleen and thymus of mice were taken out and the organ indexes were calculated.Contents of IgG,IgA,and IgM in serum were measured as well.【Result】 Every dose level of S.mussotii Franch could effectively prevent immunological liver injury in mice by affecting the contents of IgG,IgA,and IgM,adjusting the disorder of B lymphocytes network,and converting abnormal immune status to normal.【Conclusion】 Preventive administration of S.mussotii Franch could alleviate the damage of immunological liver injury in mice,and weaken the humoral immune function of hyperthyroidism,so as to play a certain role in the capacity of liver protection,disease-resistant and anti-injury.