不同红蓝光质比LED光源对铁皮石斛试管苗生长的影响

Effects of light emitting diode (LED) with different red/blue quality ratios on the growth of Dendrobium officinale plantlets in vitro

【目的】研究不同红蓝光质比LED光源对铁皮石斛试管苗生长的影响,为高品质的铁皮石斛试管苗生产提供理论依据,也为LED光源在植物组培中的应用和经济可行性分析提供参考。【方法】以铁皮石斛试管苗为试材,在LED光源的红光100%R(主波长640nm),蓝光100%B(主波长464nm),80%R+20%B,70%R+30%B,50%R+50%B 5种不同光质配比下培养,以普通荧光灯(PGFL)作为对照,比较经不同光质比处理90d后试管苗的形态指标、叶绿素、可溶性糖、可溶性蛋白含量以及根系活力。【结果】铁皮石斛试管苗株高在100%R处理下达到最大值;叶数、根长均在80%R+20%B处理下达到最大值;根数在70%R+30%B处理下达到最大值。在50%R+50%B处理下,铁皮石斛试管苗的干鲜质量、叶绿素a、叶绿素b、叶绿素(a+b)、可溶性糖含量和根系活力均达到最大值。可溶性蛋白在100%B处理下达到最大值。【结论】红蓝光比例为1∶1时有利于铁皮石斛试管苗的生长、叶绿素合成及干物质和糖的积累。

【Objective】 The effects of LED with different red/blue quality ratios on the growth of Dendrobium officinale plantlets in vitro were studied.【Method】 The Dendrobium officinale plantlets in vitro were cultured under LED with five different ratios（100%R（red）,100%B（blue）,80%R＋20%B,70%R＋30%B,50%R＋50%B）,and PGFL was used as control.After 90 days,the effects of different treatments on morphology,chlorophyll content,soluble sugar content,soluble protein content,and root activity of Dendrobium officinale plantlets in vitro were examined.【Result】 The plant height of Dendrobium officinale plantlets in vitro reached maximum under 100%R.The leaf number and root length reached maximum under 80%R＋20%B.The root number reached maximum under 70%R＋30%B.The dry and fresh weight,chl a content,chl b content,chl a＋b content,soluble sugar content,and root activity all reached maximum under 50%R＋50%B,and the soluble protein content reached maximum under 100%B.【Conclusion】 The LED with red/blue ratio of 1∶1 had the best effects on growth,synthesize chlorophyll,accumulate dry matter and sugar of Dendrobium officinale plantlets in vitro.