慢性乙型肝炎和肝癌患者ICAM-1表达及细胞因子对其调节作用的观察

Expression of ICAM-1, being regulated by cytokine in patients with chronic hepatitis B and hepatocellular carcinoma

目的 研究细胞间粘附分子 - 1(ICAM- 1)在乙型肝炎 (乙肝 )病毒清除及肝癌免疫机制中的意义及细胞因子对 ICAM- 1的调节作用。方法 利用免疫组化技术对 HBV相关肝病肝组织ICAM- 1表达进行检测 ,同时检测乙肝组织内 HBc Ag表达状况。利用细胞酶联免疫技术观察 IFN-r、IFN- α、TNF对人肝癌细胞 H- 740 2 ICAM- 1表达的调节作用。结果 在慢性乙肝中 ,ICAM- 1表达愈强 ,肝内 HBc Ag表达愈少 ;肝癌 ICAM- 1表达较癌旁及肝硬化组织减弱 ;细胞因子对 ICAM- 1表达有增强作用。结论 慢性乙肝组织 ICAM- 1一定程度的有利于 HBV清除 ;ICAM- 1表达减少可能是肝癌发生过程中免疫逃逸的重要机制 ;IFN- r、IFN- α、TNF对 ICAM- 1表达有增强作用。

Objective To explore the significance of ICAM-1 in the mechanism of HBV clearance in hepatitis B and in the development of hepatocellular carcinoma(HCC).In the meantime, to study the immune modulation of cytokines to ICAM-1.Methods Expression of ICAM in liver specimens from patients with HBV-related liver diseases were detected by immunohistochemical method, HBcAg were also detected in CHB.The hepatoma cell line H-7402 was used to observe the influnce of several cytokines such as IFN-r, IFN-α, TNF on the ICAM-1 expression in vitro. Results In hepatitis B, the more strongly ICAM-1 was expressed,the more weakly HBcAg expression within liver was showed;ICAM-1 expession was weaker in HCC than that in liver cirhosis and pericarcinorma; ICAM-1 was weakly expressed in hepatoma cell line H-7402 and increasly expressed when induced by IFN-r,IFN-a,TNF.Conclusions ICAM-1 expression in liver of hepatitis B might contribute to HBV clearance. The decrease of ICAM-1 expression might be related to development of HCC by immune escape mechanism. Cytokines such as IFN-r, IFN-α, TNF can up-regulate ICAM-1 level. [