新疆维、哈两民族人群血浆中谷氨酰胺的含量及其与2型糖尿病关系的研究

The glutamine content in plasma and it'is relationship with Type 2 diabetes in Uyghurq and Kazak populations

目的探讨谷氨酰胺与2型糖尿病的关系。方法采用高效液相色谱仪(HPLC)来测定新疆维、哈两民族血浆中谷氨酰胺的含量;使用放免法测定空腹血清胰岛素(FINS)含量;采用全自动生化分析仪来测定空腹血清中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDLC)、低密度脂蛋白(LDLC)的含量。结果维吾尔族正常人与糖尿病患者之间血浆中谷氨酰胺含量差异有统计学意义(P<0.01);哈萨克族正常人与糖尿病患者之间血浆中谷氨酰胺含量差异无统计学意义;维哈两民族的正常人血浆中谷氨酰胺含量差异有统计学意义(P<0.01);维哈两民族的糖尿病患者血浆中谷氨酰胺的含量差异有统计学意义(P<0.05)。维哈两民族人群血浆中的谷氨酰胺含量与空腹血糖(FBG)水平呈负相关(r=-0.4858,P<0.001),与FINS水平呈正相关(r=0.236,P<0.05),与胰岛素分泌指数(HOMA-IS)呈正相关(r=0.52,P=0.001),与TG呈负相关(r=-0.4330,P<0.01),与LDLC呈负相关(r=-0.4077,P<0.01),与HDLC呈正相关(r=0.2553,P<0.01)。结论谷氨酰胺可能是2型糖尿病的一个保护性的影响因素。

Objective To investigate the relationship between glutamine and Type 2 diabetes （T2DM）. Methods High Per- formance Liquid Chromatography （HPLC） was used to determine the contents of glutamine in plasma of Uygur and Kazak ethnic popula- tions. Radioimmunoassay was used to determine fasting serum insulin （FINS）. Automatic biochemical analyzer was used to determine TC, TG, HDL and LDLC. Results The glutamine contents of healthy Uygur people was significantly different from those with T2DM （P 〈 0.01 ） , but there was no such a significant difference in Kazak people. Whether healthy （P 〈 0.01 ） or with T2DM （P 〈 0.05 ）, there was significant difference in plasma glutamine contents between Uygur and Kazak people. Glutamine content was negatively correla- ted with FBG （r = -0. 4858, P 〈 0. 001 ）, TG （r = -0. 4330. P 〈 0. 01 ）, LDLC （r = -0. 4077,P 〈 0. 01 ）, but positively correlated with FINS（r =0. 236,P 〈0. 05） ,HOMA-IS（r =0. 52,P =0. 0001 ） ,HDLC（r =0. 2553 ,P 〈0. 01 ）. Conclusion Glutamine maybe a protec- tive factor to type 2 diabetes.