高血糖对大鼠肾皮质葡萄糖转运蛋白1 mRNA表达的影响

The effect of hyperglycemia on the expression of glucose transporter I mRNA in renal cortex of rats

【摘要】目的探讨葡萄糖转运蛋白1（glucosetransporter1，GLUT1）在糖尿病。肾病（diabeticnephropathy，DN）发病机制中的作用。方法将36只Wistar大鼠随机分为对照组10只、高血糖组及2型糖尿病组各13只，分别采用腹腔内注射链脲佐菌素及高脂饮食加腹腔内注射链脲佐菌素方法制备高血糖及2型糖尿病模型。10W末实验结束后，取大鼠肾皮质，同时检测大鼠体重、肾重、肾重百分比，血清BUN；采用逆转录聚合酶链反应半定量分析肾皮质GLUT1 mRNA的表达。结果高血糖组大鼠的体重显著降低，肾重、肾重百分比、BUN、FPG和ISI水平均高于2型糖尿病组及对照组．差异均有统计学意义（P〈0．05）。而FINS的水平低于2型糖尿病组及对照组，差异亦均有统计学意义（P均〈0．05）。高血糖组和2型糖尿病组GLUTlmRNA在‘肾皮质中的表达均显著高于对照组．且高血糖组GLUT1mR．NA在肾皮质的表达显著高于2型糖尿病组（P均〈0．05）。结论GLUT1在肾皮质中有表达．高血糖可以上调肾皮质GLUT1表达，GLUT1可能与DN的发病相关。

Objective To investigate relationship between glucose transporter 1 （GLUT1） mRNA and diabetic nephropathy （DN）. Methods 36 healthy male Wistar rats weighing 180~220 g were randomly divided into three groups, normal control group （10 cases）, type 2 diabetic rat model （13 cases）, and high- glucose rat model （13 cases）. Type 2 diabetic rat model were induced by intraperitoneally administered low dose of streptozocin and fed with food of high fat. High glucose rat model were induced by intraperitoneally ad- ministered high dose of streptozocin in rats and fed with normal food. After ten weeks, the renal cortex of rats were extracted,body weight, the kidney weight, the kidney weightxl00/the body weight and BUN were de- tected. Semiquantitative analysis on the expression of GLUT1 mRNA in renal cortex were investigated by re- verse transcriptase polymerase chain reaction. Results The body weight of rats in high glucase group were signifi- cance induced. The kidney weight, kidney weight percentage, BUN, FPG levels and ISI in high glucose group were all higher than type 2 diabetic group and normal control group, FINS was lower than these two groups. The differences all had statistical significance （Pall〈 0.05）. The mRNA expressions in renal cortex of high glucose group were higher than type 2 diabetic group and normal control group, and the differences all had statistical significance（Pall〈 0.05）. Conclusion GLUT1 expressed in renal cortex. Hyperglycemia can increase the expression of GLUT1 mRNA in renal cortex of rats,the increase of the expression of GLUT1 mRNA maybe corre- late with DN.