食管癌患者临床病理特征与生存素及表皮生长因子受体蛋白质表达的关系

Expression of survivin gene in esophageal carcinoma and its correlation with expression of epidermal growth factor receptor

目的研究食管鳞癌组织中生存素(survivin)基因表达和临床病理特征的关系及与表皮生长因子受体(EGFR)表达的相关性。方法 51例食管鳞癌患者术后组织蜡块,取癌旁>5cm正常食管组织蜡块45例作为对照。所有患者术前未行放、化疗,用免疫组织化学方法检测survivin和EGFR蛋白质表达情况,分析survivin基因蛋白质表达与食管鳞癌组织的分化程度、浸润深度、肿瘤长度、临床分期及淋巴结转移之间的关系及与EGFR蛋白质表达的相关性。结果食管鳞癌组织中survivin基因阳性表达率88.2%(45/51),显著高于正常食管黏膜20.0%(9/45)(P<0.01)。Survivin基因表达与食管鳞癌组织的分化程度、浸润深度、肿瘤长度、临床分期及淋巴结转移情况无明显相关性(P>0.05)。Survivin和EGFR蛋白质表达差异无统计学意义(χ2=0.444,P>0.05)。结论生存素与EGFR在食管癌发生发展过程中可能有协同作用。

Objective The study aimed to study the expressions of survivin gene in squamous esophageal carcinoma and its correlation with the expressions of epidermal growth factor receptor （EGFR）. Methods The expression of survivin and EGFR protein in 51 paraffin block samples of esophageal squamous carcinoma were detected by immunohistochemistry technology and 45 normal esophageal tissues which were taken from the areas 〉 5 cm from the cancer as non-tumor control samples. All patients did not accept radiotherapy and chemotherapy before operation. The relationship of survivin expression and tumor differentiation degrees, invasion depth, tumor size, lymph node metastasis,clinical stage and its correlation with the expressions of EGFR gene were analyzed. Results The expression of survivin in esophageal squamous carcinoma was 88.2% （45/51）, which was obviously higher than that of normal esophageal epithelium 20.0 % （9/45）（ P d0.01）. There were no relationship between survivin gene expression and the tumor size,invasion depth, tumor differentiation degrees,lymph node metastasis and clinical stage（ P 〉0.05）. There was no significant difference between survivin and EGFR expression in esophageal squamous carcinoma （χ2 = 0. 444, P 〉 0.05）. Conclusion Survivin and EGFR favored development and progress of the malignant change in esophageal squamous carcinoma.