肝癌组织中c-myc基因突变的研究

Mutation research of c-myc gene in hepatocellular carcinoma

目的检测肝细胞癌中c-myc的DNA苏氨酸58(T58)和丝氨酸62(S62)的突变情况,以揭示肝癌发生发展的机制。方法应用聚合酶链反应(PCR)方法,检测89例肝癌组织及癌旁组织中c-myc基因的T58和S62的突变情况。结果在89例肝癌组织及癌旁组织中未见c-myc的T58或S62及周围氨基酸突变,但发现c-myc的DNA水平与原发性肝细胞癌(HCC)的病理分级存在一定关系,低分化组(87.5%,21/24)、中分化组(74.4%,29/39)及高分化组(53.8%,14/26)与c-myc阳性率差异有统计学意义(P<0.05)。结论 c-myc基因的表达与HCC的病理分级存在一定关系,其在肝癌组织的高表达不是由于T58或S62的突变导致c-myc稳定性增高所致,亦有其他的因素参与其中。

Objective To detect the mutations of T58 and $62 in c-myc gene in hepatocellular carcinoma（HCC） tissues,and explore the mechanism in the occurrence and development of HCC. Methods Polymerase chain reaction method was employed to test the mutation of T58 and $62 in c myc gene in 89 HCC tissues and their corresponding para-neoplastic tissues. Results No mutations of T58 and $62 were found in c-myc gene in HCC tissues and their corresponding para-neoplastic tissues, but the DNA level of c-myc was correlated with the histological differentiation of the HCC samples in the comparisons among poorly differentiated（87.5 %,21/24）, moderately differentiated（74.4%, 29/39） and well differentiated group（53.8%, 14/26）（ P 〈0.05）. The further comparison in c-myc between poorly differentiated and well differentiated showed significant difference （P 〈 0.05 ）. Conclusion There is certain relationship between the DNA level of c-myc and the pathological classification of the HCC samples, the over- expression of c-myc protein in HCC may contribute to the other factors otherwise the mutations of T58 and $62 in c- myc gene.