背根神经节组织中GAP-43基因克隆

GENE CLONING OF GAP-43 FROM RAT DORSAL ROOT GANGLIA

目的 :克隆新生大鼠背根神经节组织中生长相关蛋白 - 43(GAP- 43)基因。方法 :采用 RT- PCR法 ,从新生大鼠背根神经节组织 m RNA中扩增 GAP- 43基因 CDS区片段 ,克隆入 T载体 ,并经序列测定。结果 :RT-PCR法扩增出一特异产物与预期长度 778bp相符 ,T载体克隆测序与 GAP- 43基因 10 0 %同源。结论 :采用 RT-PCR和 T载体技术获得了新生大鼠背根神经节组织中的 GAP- 43基因克隆。

Objective: To obtain the gene cloning of GAP-43 from rat dorsal root ganglia(DRG).Methods: The CDS of GAP-43 was amplified by RT-PCR from rat DRG, and then was ligated into pGEM-T vector. The DNA sequence was detected.Results: The product of RT-PCR was the 778bp which matched the size of purpose. The sequence of GAP-43 was completely homogeneous with the GAP-43 sequence reported.Conclusions: GAP-43 gene had been cloned from rat DRG using RT-PCR and T vector techniques.