高聚金葡素局部治疗浅表转移性肿瘤的近期疗效及对免疫功能的影响

The treatment effects and immune function of Highly Agglutinative Staphylococci local therapy in patients with superficial matastatic tumor.

目的 评价高聚金葡素 (HAS)局部治疗浅表转移性肿瘤的近期疗效及对机体免疫功能的影响。方法 对35例浅表转移性肿瘤患者实施局部治疗 ,HAS10 0 0 U瘤体内注射 ,每日 1次 ,总疗程 30天。采用流式细胞术检测淋巴细胞亚群及 CD2 8+ 淋巴细胞 ,CD96 L+ 淋巴细胞。采用 MTT法检测淋巴细胞杀伤活性 ,并作淋巴细胞杀伤活性与 CD96 L+ 淋巴细胞变化的相关分析。结果 CR1例、PR10例、MR18例、CS6例 ,总缓解率为 31.4% ,总有效率为 82 .9%。治疗后较治疗前患者淋巴细胞增多 (P<0 .0 5 ) ,尤以 T8+ 淋巴细胞增多明显 (P<0 .0 1) ,淋巴细胞杀伤活性增高 (P<0 .0 5 ) ,而 B细胞、NK细胞无明显变化 (P>0 .0 5 ) ,淋巴细胞杀伤活性与 CD96 L+淋巴细胞变化呈正相关 (r=0 .840 ,P<0 .0 1)。结论 HAS治疗浅表转移性肿瘤近期疗效明显 ,能增强机体细胞免疫功能 ,fasl- fas死亡通道可介导淋巴细胞的细胞毒性。

Objective [WT5”BZ]To evaluate treatment effects of HAS local therapy in 35cases with superficial metastatic tumor.[WT5”HZ]Methods [WT5”BZ]HAS 1000U perday was injected into tumor for 30 days.Subsets of lymphocytes,CD 28 k+lymphocyte and CD 96 L +lymphocyte were detected by flow cytometry.Cytotoxicity of lymphocyte was measured by MTT assay.Correlation analysis of lymphocyte cytotoxicity with CD 96 L +lymphocyte was made.[WT5”HZ]Results [WT5”BZ]CR was seen in l case,PR in 10 cases,MR in 18 cases,SD in 6 cases.Increased T lymphocytes specially CD 8 +lymphoctye and higher cytotoxicity of lymphocytes were shown.But no difference in B lymphocytes and NK cells was seen.The increased cytotoxicity of lymphocytes was closely correlated with increased CD 96 L +lymphocytes.[WT5”HZ]Conclusions [WT5”BZ]Local injection of HAS can be used as therapy for patients with superficial matastatic tumor.The increased cellular immune function was shown after trentment.The cytotoxicity of lymphocytes may be mediated by fasL-fas death Path. [WT5”HZ]