肝素对大鼠Ⅱ度烧伤创面愈合的影响

THE EFFECT OF HEPARIN ON WOUND HEALING OF SECOND-DEGREE BURNED RATS

目的 研究肝素对大鼠 度烧伤创面愈合的影响。方法 2 0只 SD大鼠随机分为两组 ,制成2 0 %面积深 度烧伤 ,实验组采用肝素 10 0 U /kg加生理盐水至 1ml皮下注射 ;对照组采用生理盐水 1ml皮下注射 ,每天一次直至创面完全愈合。从大体观察创面愈合天数及全身有无出血现象 ,光镜观察肉芽组织及胶原纤维生长情况 ,电镜观察成纤维细胞生长情况。结果 大鼠全部成活。实验组创面愈合时间为 (2 2 .8± 1.87)天 ,对照组为(2 6 .2± 2 .82 )天 ,实验组创面愈合所需时间明显少于对照组 (P<0 .0 0 5 )。光镜观察发现实验组肉芽组织及胶原纤维生长明显优于对照组。电镜观察发现实验组成纤维细胞生长优于对照组。结论 肝素皮下注射能加速创面愈合。

Objective To study the effect of therapeutic effect of heparin on wound healing of second-degree burned rats. Methods Deep second-degree burns with 20% of total body surface were made in 20 rats, which were randomly divided into 2 groups. The experimental group was treated subcutaneously injection with 1ml of heparin 100 U/kg and saline solution, and the control group was treated with 1ml of saline solution, once a day until complete healing of the burned wound. The healing time were compared, the growth of granulation tissue and collagen fibers were evaluated under light microscope, and the growth of fibroblasts were observed under electronic microscope. Results All rats survived, the healing time of experimental group(22.8±1.87 days) were much shorter than that of control group (26.2±2.82 days) ( P <0.005). Light microscope observation showed that the growth of granulation tissue and collagen fibers of experimental group were much better than that of control group, and electronic microscope also showed that the fibroblast growth was obviously better in the experimental group. Conclusion Subcutaneous injection of heparin can promote wound healing.