碱性成纤维细胞生长因子和转化生长因子-β在溃疡与增生性瘢痕组织中的表达及其对创面修复的影响

CHARACTERISTICS OF bFGF AND TGF-β EXPRESSION IN DERMAL CHRONIC ULCERS AND HYPERTROPHIC SCARS AND THEIR EFFECTS ON TISSUE REPAIR

目的 研究碱性成纤维细胞生长因子 (b FGF)与转化生长因子 - β(TGF- β)在溃疡和增生性瘢痕组织中的表达特征以及与修复结果的关系。方法 2 1例标本包括体表慢性溃疡 8例、增生性瘢痕 8例和正常皮肤5例。用免疫组织化学法和常规病理技术确定两种生长因子在溃疡和增生性瘢痕的定位与表达量。结果 b FGF和TGF- β在正常皮肤中有少量表达 ,主要位于表皮基底细胞胞浆和细胞外基质。此外 ,b FGF还见于真皮毛细血管内皮细胞等。在溃疡组织 b FGF与 TGF- β表达量明显增加 ,其中 b FGF的阳性信号主要见于成纤维细胞和毛细血管内皮细胞 ,而 TGF- β则仅见于炎性细胞。在增生性瘢痕 TGF- β的表达为阴性 ,而 b FGF则仍呈现出较高的表达量。结论 在高浓度生长因子环境下创面修复延迟可能和生长因子与其受体结合障碍有关。研究结果还提示 b FGF参与了瘢痕发生的全过程 ,TGF- β则主要作用于瘢痕形成早期。

Objective To localize the distribution of basic fibroblast growth factor (bFGF) and transforming growth factor-β(TGF-β) in tissues from dermal chronic ulcer and hypertrophic scar and to explore their effects on tissue repair. Methods Twenty-one cases were detected to localize the distribution of bFGF and TGF-β, among them, there were 8 cases with dermal chronic ulcers, 8 cases with hypertrophic scars, and 5 cases of normal skin. Results Positive signal of bFGF and TGF-β could be found in normal skin, mainly in the keratinocytes. In dermal chronic ulcers, positive signal of bFGF and TGF-β could be found in granulation tissues. bFGF was localized mainly in fibroblasts cells and endothelial cells and TGF-β mainly in inflammatory cells. In hypertrophic scar, the localization and signal density of bFGF was similar with those in granulation tissues, but the staining of TGF-β was negative. Conclusion The different distribution of bFGF and TGF-β in dermal chronic ulcer and hypertrophic scar may be the reason of different results of tissue repair. The pathogenesis of wound healing delay in a condition of high concentration of growth factors may come from the binding disorder of growth factors and their receptors. bFGF may be involved in all process of formation of hypertrophic scar, but TGF-β may only play roles in the early stage.