碱性成纤维细胞生长因子对成骨细胞粘附特性影响的实验研究

THE INFLUENCE OF BASIC FIBROBLAST GROWTH FACTOR ON THE ADHESION CHARACTERISTICS OF OSTEOBLASTS IN RABBIT

目的 针对骨组织工程中成骨细胞与基质材料间促粘附问题 ,研究碱性成纤维细胞生长因子(b FGF)对成骨细胞粘附特性的影响。方法 取兔骨髓基质干细胞来源的成骨细胞体外培养 ,分别用 5、10、5 0、10 0及 2 0 0 ng/ ml浓度的 b FGF诱导培养 2 4小时作为实验组 ;不含 b FGF的培养基作为对照组。观察接种后 0 .5、1、2、4及 8小时各时间点成骨细胞粘附情况 ,体视学计量粘附细胞数量。结果 10 ng/ ml b FGF诱导后 ,细胞粘附细胞数增加最明显 ,显著高于对照组 (P<0 .0 1)。随着 b FGF浓度增加 ,细胞粘附数并不相应增加 ,10 0 ng/ m l b FGF诱导后 ,细胞粘附数与对照组无明显差异 ;2 0 0 ng/ m l b FGF诱导后 ,细胞粘附数显著低于对照组 (P<0 .0 1)。结论b FGF具有调节成骨细胞粘附特性的作用 ,10 ng/ m l的 b FGF诱导可促进成骨细胞与基质材料间粘附 ,2 0 0 ng/ ml的 b FGF诱导不利于成骨细胞粘附。该结果对骨组织工程研究中 ,促进成骨细胞与基质材料间粘附具有重要意义。

Objective To investigate the influence of basic fibroblast growth factor (bFGF) on adhesion characteristics of osteoblasts, aimed at the important problem in bone tissue engineering of how to promote the adherence of osteoblasts to extracellular matrix materials. Methods 5 ng/ml, 10 ng/ml, 50 ng/ml, 100 ng/ml, 200 ng/ml bFGF were used to induce bone marrow stromal-derived osteoblasts of rabbit for 24 hours before incubation,and the common culture medium as the control. The attached cells were calculated with stereology method at 0.5 hour, 1st hour, 2nd hour, 4th hour, 8th hour after seeding. Results The number of attached cells was significant higher in the experimental group when induced by 10 ng/ml bFGF than that in the control group ( P <0.01); the number did not increase with the increase of bFGF concentration and there was no significant difference between the experimental group induced by 100 ng/ml bFGF and control group, and the number was even obviously lower in the experimental group when induced by 200 ng/ml than the control group( P <0.01). Conclusion bFGF can influence the adhesion characteristics of osteoblasts, 10 ng/ml bFGF can promote the adherence of osteoblasts to matrix materials, but 200 ng/ml bFGF may inhibit cell adhesion.