中药脑肺康对地鼠实验性肺气肿及肺动脉高压的防治作用

Preventive and Therapeutic Effect of Chinese Herbs Naofeikang on Experimental Emphysema and Pulmonary Artery Hypertension in Hamsters

目的 观察中药脑肺康对缺氧性肺动脉高压和肺气肿的防治效果并探讨其作用机制。方法 以弹性蛋白酶溶液滴注入金黄地鼠气管内 ,正常饲养 30 d后 ,常压下缺氧 15 d,分别设立中药脑肺康预防组、治疗组、肺气肿 +缺氧组及正常对照组。于处死动物前 ,测量平均肺动脉压 ,取静脉血进行循环内皮细胞计数 ;处死后测量右心肥大指数 ,光镜下计数左侧肺支气管肺泡灌洗液内细胞总数 ,对右侧肺进行光镜图像分析。结果 中药脑肺康预防组和治疗 3个月组平均肺动脉压、循环内皮细胞数、支气管肺泡灌洗液细胞总数、肺泡平均内衬间隔和肺细小动脉平均中膜面积百分比均明显低于肺气肿 +缺氧组 (P<0 .0 5或 P<0 .0 1)。结论 中药脑肺康可降低肺动脉压 ,保护血管内皮细胞及减轻肺内炎症反应 ,减缓肺气肿病变的发展 ,减轻缺氧造成的肺血管重建。

Objective To observe preventive and therapeutic effect of Chinese herbs Naofeikang on hypoxic pulmonary artery hypertension of elastase induced emphysema hamsters and investigate its mechanism. Methods Hamsters for 30 days after intratracheally instilled elastase, were kept in hypoxia environment under normal atmospheric pressure for 15 days, 50 hamsters were divided into prevention group (Prv), treatment group (T), emphysema+hypoxia (EH), and control group(N). Mean pulmonary artery pressure (MPAP) was measured before hamsters were killed. And then right ventricle hypertrophy index (RVHI) was measured, as well as circulating endothelial cells (CEC) and cells recoveries from bronchoalveolar lavage fluid (BALF) were counted. Meanwhile, pulmonary tissue changes were studied under light microscope with morphometric analysis. Results Compared with EH group, MPAP,CEC, and cells recoveries of BALF of Prv and T groups were significantly decreased (P<0 05 or P<0 01); Mean linear intercept and percentage of arterial media area of Prv and T groups had totally a significant difference (P<0 01) in comparison with EH group. Conclusions Chinese herbs Naofeikang could lower pulmonary hypertension, preserve vessel endothelial cells and lessen the inflammatory reaction in pulmonary tissue. Thereby, it could hinder the further development of emphysema and inhibit the remodeling of pulmonary small artery.