一种快速检测氟喹诺酮类药物的胶体金试纸卡的研制

Study on gold immunochromatography assay for rapid detection of fluoroquinolones

利用胶体金免疫层析技术研制了一种快速检测牛奶中的氟喹诺酮类药物试纸卡,并配合胶体金试纸卡读数仪使用。结果表明,该试纸卡对恩诺沙星、沙拉沙星、双氟沙星、氧氟沙星、诺氟沙星、环丙沙星、培氟沙星、氟甲喹、达氟沙星的检测限为20μg/L,对依诺沙星、恶喹酸的检测限为40μg/L,检测时间为10 min,假阳性率和假阴性率均为0。该试纸卡能够准确、可靠、简便地检测牛奶中残留的氟喹诺酮类药物,适合大量样品的现场检测。

To establish a rapid assay for detecting fluoroquinolones in milk, gold immunochromatography assay was studied, which was used with a readout instrument？ of colloidal gold test paper card together. The limit of detection （enrofloxacin, sarafloxaxcin, difloxacin, ofloxacin, norfloxacin, ciprofloxacin, pefloxaciu, flumequine, danofloxacin） is 20 μg/L, and （enoxacin, oxolinic acid） is 40 μg/L. And we can obtain the results in 10 minutes. The assay gives no false positive and false negative results. The method is accurate, simple, reliable and convenient to rapidly detect fluoroquinolones of a lot of samples on the spot.