摘要
以还原法为理论基础,研究了嗜热链球菌数的快速计数方法。借助细菌总数快速检测仪对加入指示剂后的样品颜色(RGB)变化进行检测,建立了色差值B值与嗜热链球菌数目之间的标准方程,相关系数可达到0.9820。对标准曲线进行准确度分析,与国标计数法检测结果差异不显著。结果表明,色差分析法是嗜热链球菌数快速计数的有效方法,该方法具有操作简单,耗时短等优点。
The method for rapid detecting the bacterial count of Streptococcus thermophiles was studied based on reduction method as the theo- retical bash. The apparatus detected continuously the color(R.GB) of the samples which had been added indicator, A standard curve was estab- lished to show the relation between color variation B and count of Streptococcus thermophiles, with the coet^icient of correlation of 0.9820. There was no significant difference between the two results of the color variation analysis and the GB counting method. The results showed that color variation analysis was appropriate for the rapid detecting the bacterial count of Streptococcus thermophiles. The method has advantages of convenient operation, time saving, ect.
出处
《中国乳品工业》
CAS
北大核心
2013年第8期55-57,共3页
China Dairy Industry
基金
教育部长江学者和创新团队发展计划(IRT-0959-304)
关键词
色差分析法
嗜热链球菌
菌数
细菌总数快速检测仪
color variation analysis
Streptococcus thermophiles
bacterial count
total bacteria measuring apparatus