百合愈伤组织的诱导及瞬时表达的研究

Studies on Callus Induction and Transient Expression of Lilium tenuifolium (Oriental×Trumpet)

以百合品种‘罗宾娜’(Robina)鳞片、花丝和花梗分化的不定芽为试材,诱导愈伤组织,并通过固体、液体和经过液体预培养后在固体上培养的方式继代增殖。结果表明:分化的不定芽在萘乙酸(NAA)0.5mg/L的培养基上愈伤组织的诱导率最高;从芽基部形成的愈伤组织以经过液体预培养后在固体上培养的方式继代,繁殖速率最大,再生率为100%。使用农杆菌介导转化pCAMBIAl301质粒后,3种不同方式培养的愈伤组织的GUS瞬时表达效率差异并不显著。

Taking Lilium tenuifoliu Oriental×Tumpet ＇Robina＇ as materials,and adventitious buds which differentiated from bulb scales,filaments and stalk of it were used to form embryogenic callus,multiplication was conducted via solid,liquid and liquid preculture then solid culture methods.The results showed that it produced the most embrogenic callus when explants were cultured on induction medium which contained 0.5 mg/L NAA.The best result of callus proliferation was made via a alternative culture method which callus was transferred on solid and liquid medium by turns.The GUS gene contained in vector pCAMBIA1301 was introduced into the embryogenic cultures by Agrobacterium-mediated tracient expression and then GUS chemical tissues staining was conducted to observe.The results showed that it was no significant transient expression efficiency variance among callus of three cultivation methods.