海南木莲遗传多样性的ISSR及亲缘关系的分析

Studies on the Genetic Diversity and Relationship of Manglietia hainanensis Dandy by ISSR

将简单重复序列区间扩增多态标记(inter-simple sequence repeats,ISSR)技术应用于海南木莲4个种群的36份样品的遗传多样性分析,10个引物共扩增出106条条带,其中有87条多态性条带,多态位点百分率(PPB)为82.08%。4个种群的海南木莲等位基因数(Ne)的变异幅度为1.416 6-1.625 7,Nei’s基因多样性指数(H)的变异幅度为0.222 8-0.339 2,Shannon信息指数(I)的变异幅度为0.315 1-0.491 0,种群间的分化系数(Gst)为0.197 1,由遗传一致度进行了4个种群的UPGMA聚类。

Inter-simple sequence repeat（ISSR）technique was applied to detect 4 populations and 36 samples of Manglietia hainanensis Dandy,and 106 bands were amplied by 10 primers,including 87 polymrphic bands.The polymorphic percentage is 82.08%.The variation range of alleles per locus（Ne）was 1.416 6-1.625 7;variation range of Nei’s gene diversity（H）was 0.222 8-0.339 2;variation range of shannon’s information index（I）was 0.315 1-0.491 0;coefficient of gene differentiation（Gst）of the 4 populations was 0.197 1.Based on the genetic identity,dendrogram of 4 populations was constructed by UPGMA.