香蕉枯萎病拮抗放线菌1-g-59的筛选与鉴定

Screening and Identification of Antagonistic Actinomyces 1-g-59 Against Fusarium oxysporium f. sp. cubense

通过对海南美台、南宝蕉园香蕉根际土壤的分离和筛选,获得5株对FOC 4具有拮抗作用的放线菌,其中以菌株1-g-59活性最强。根据进行形态特征、培养特征、生理生化特征、细胞壁化学成分分析、16S rDNA序列和系统发育分析等研究,鉴定菌株1-g-59为Streptomyces lunalinharesii。盆栽试验结果表明,菌株1-g-59发酵液对香蕉枯萎病具有较强的防治作用,防治效果达64.9%。

Five antagonistic strains against Fusarium oxysporumf.sp.cubense were isolated and screened from the rhizosphere soil of the banana garden in Meitai and Nanbao,strain 1-g-59 possessed the most prominent bioactivity.By studying the morphology,cultural,physiological and biochemical properties,cell wall components analysis,16S rDNA and phylogenetic tree,the results showed that strain 1-g-59 was identified as Streptomyces lunalinharesii.Pot experiment showed that control effects of the fermentation filtrate of strain 1-g-59 on banana fusarium wilt disease was 64.9%.