响应面优化虾夷扇贝生殖腺多肽-Ca^(2+)螯合物制备工艺

Optimization of Preparation of Peptide-Ca^(2+) Complex from Scallop (Patinopecten yessoensis) Gonad

优化虾夷扇贝生殖腺多肽-Ca2+螯合物的制备工艺,在单因素试验的基础上,应用响应面二次回归中心旋转组合试验设计,分析了反应pH值、时间、温度和多肽-氯化钙质量比对螯合物得率的影响。结果表明:多肽与Ca2+螯合的最优工艺条件为pH8.0、温度64.8℃、反应时间22min、质量比3.4:1,在此条件下,螯合物得率预测值为6.78%,实际值为6.81%,该螯合物中氮和钙质量分数分别为(6.67±0.11)%和(9.75±0.17)%。红外光谱分析表明,多肽中的氨基和羧基可能均参与了Ca2+的螯合反应。体外模拟胃肠道消化后的钙透析率为(41.86±2.33)%,但与未消化对照组无显著差异(P>0.05)。因此,虾夷扇贝生殖腺多肽-Ca2+螯合物具有较好的钙生物利用度。

Response surface methodology based on a quadratic central composite rotary design was used to optimize the preparation of peptide-Ca2＋ complex from scallop（Patinopectenyessoensis） gonad.The influence of pH,time,temperature,and peptide/calcium ratio on the yield of peptide-Ca2＋ complex was investigated.The results showed that the optimum reaction conditions for forming Ca2＋ chelating peptides were pH 8.0,22 min,64.8 ℃,and peptide/calcium ratio 3.4：1.Under these conditions,the predicted yield was 6.78% while the actual yield was 6.81%.The complex contained（6.67 ± 0.11）% nitrogen and（9.75 ± 0.17）% calcium.Infrared spectroscopic study revealed that both amino and carboxyl groups were involved in the complex.Calcium dialyzability of the complex after in vitro digestion was（41.86 ± 2.33）%,but showed no significant difference from control（P 0.05）.Therefore,scallop（Patinopectenyessoensis） gonad hydrolysate-Ca2＋ complex might have good calcium bioavailability.