摘要
为优化碱提酸沉法制备酸枣仁蛋白的工艺条件,通过单因素试验考察了液料比、pH值、浸提温度和浸提时间对酸枣仁蛋白提取率的影响,在单因素试验的基础上,采用Box-Behnken设计建立并分析各因素与提取率之间关系的数学模型。结果表明:碱提酸沉法制备酸枣仁蛋白的最佳工艺条件为液料比24:1(mL/g)、pH11、提取温度57℃、提取时间46min,在此条件下酸枣仁蛋白提取率的最大理论值为73.15%,实际蛋白提取率为73.11%,该回归模型具有较好的预测性能,可用于指导生产实践。
The extraction condition of protein from Zizyphi spinosi Semen by alkaline extraction and acid precipitation was optimized by response surface methodology.The extraction efficiency of protein was investigated with respect to four variables including solvent/solid ratio,pH,temperature and time.A mathematical model was fitted by means of BoxBehnken design.The optimum conditions for extracting protein from ZizyphispinosiSemenwere determined as 24：1（mL/g）,11,57 ℃ and 46 min for solvent/solid ratio,pH,temperature and time,respectively.Under these conditions,the maximum theoretical extraction yield of protein was 73.15%,which closely agreed with the actual value of 73.11%.Thus,the model developed in this study has good prediction capability.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第16期134-138,共5页
Food Science
关键词
酸枣仁
蛋白
碱提酸沉法
响应面法
Zizyphi spinosi Semen
protein
alkaline extraction and acid precipitation method
response surface methodology
