PCR及核糖体基因分型法检测和鉴定脂环酸芽孢杆菌

Detection and Identification of Alicyclobacillus by Use of PCR and Automated Ribotyping

为实现对果汁生产中脂环酸芽孢杆菌从原料到成品的快速检测和鉴定,采用聚合酶链式反应(PCR)以及自动化核糖体基因分型RiboPrinter,对经过分离、筛选和纯化后的59株分离菌株进行鉴定。结果表明:自动化核糖体基因分型使得鉴定结果更加快速、准确和可靠,最大化避免了其他因素对实验的影响,且在鉴定到种的基础上可以进一步分类,进行溯源分析。RiboPrinter分型结果为建立我国脂环酸芽孢杆菌分子分型数据库积累了一定的数据和经验,但由于菌株的来源地及数量有限,因此尚不能看出核糖体基因型是否有明显的地域分布特征。

A rapid detection and identification method for Alicyclobacillus was proposed by combined use of PCR and automated ribotyping（RiboPrinter）.A total of 59 pure strains were isolated and identified by the method.The results showed that automated ribotyping allowed more rapid,accurate and reliable identification with maximum interference avoidance,the further classification of identified species and enabled their traceability.The results of this study are expected to provide useful data to establish a database for Alicyclobacillus typing.However,it remains unclear that whether ribotyping can indicate regional distribution characteristics of Alicyclobacillus due to limited sources and number of strains in this study.