膜渗析与离子色谱联用技术测定蜂王浆中的葡萄糖、果糖和蔗糖

Determination of Glucose,Fructose and Sucrose in Royal Jelly by Ion Chromatography Coupled with Dialysis as an Online Sample Pretreatment Technique

建立三氯乙酸法沉淀试样中蛋白,在线膜渗析与离子色谱联用技术分离测定蜂王浆中葡萄糖、果糖和蔗糖的分析方法;采用METROSEP CARB 1(150mm×4.0mm)阴离子交换分离柱和脉冲安培检测器;对仪器的色谱和渗析条件进行优化。结果表明:优化条件为选择100.0mmol/L NaOH溶液为淋洗液、淋洗液流速1.0mL/min、停留渗析时间10min、进样转移时间33s,此条件下葡萄糖、果糖和蔗糖的检出限分别为0.068、0.085mg/L和0.081mg/L,方法的回收率为85%～107%,方法适用于同时测定蜂王浆中葡萄糖、果糖和蔗糖的含量。

A method for the determination of glucose,fructose and sucrose in royal jelly using ion chromatography coupled with online dialysis following protein precipitation with trichloroacetic acid has been developed.The optimal chromatographic and dialysis conditions were investigated.The instrument consisted of a METROSEP CARB 1（150 mm × 4.0 mm） anion exchange column and a pulsed amperometric detector.Elutions were performed using 100.0 mmol/L NaOH at a flow rate of 1.0 mL/min.Dialysis time and transfer time were 10 min and 33 s,respectively.The detection limits for glucose,fructose and sucrose were 0.068,0.085 mg/L and 0.081 mg/L,respectively.The recoveries were 85%–107%.This method proved suitable for the simultaneous determination of glucose,fructose and sucrose in royal jelly.