一株石油降解菌产表面活性剂条件的优化

Optimizing Surfactant Produce Conditions for An Oil-degrading Stain

利用Minitab软件,采用Plackett-Burman设计、选择了温度、转速、pH、加油量、时间5个因素对一株石油降解菌CT-6(Pseudomonas aeruginosa)产表面活性剂的条件进行了优化。结果表明:当温度为35℃、加油量为4%、pH为8.0、转速为213 r/min、培养时间为152 h时,产生的表面活性剂排油效果最好,排油圈达6.4 cm,乳化性能达92%,细胞表面疏水性达41%,比优化前分别提高了35.9%、29.6%、64%。

The experiment was conducted with minitab software and applied Packett-Burman design with five factors, including temperature, pH, inoculate capacity, rotational speed and the initial amount of oil to optimize the surfactant produce conditions of oil degrading strain CT-6 （Pseudomonas aeruginosa）. The results showed that when the temperature was 35℃, the original amount of oil 4.0%, pH 8.0, rotational speed 213 r/min, incubation time 152 h, the activity of surfactant which produced by strain CT-6 was best, the exhibit circle was 6.4 cm, emulsification performance reached 92%, cells hydrophobic was 41%, increased by 35.9%, 29.6%, 64.0%, respectively.