组织工程甲壳素载体支架对角膜上皮损伤修复作用的研究

Effect of chitin-based tissue engineering carriers on corneal epithelial wound healing

探讨甲壳素载体支架培养角膜上皮细胞的可行性以及对角膜上皮的损伤修复作用。以自制的甲壳素膜片为材料,测定其厚度、含水量、透光率、机械强度、生物相容性和降解性,在甲壳素膜片上培养兔角膜上皮细胞观察其细胞相容性,构建组织工程角膜上皮,将其移植到受损的角膜上皮层,观察其修复作用。结果甲壳素膜厚度为0.20mm,含水量为81.59%,不同波长下的透光率均大于80%,干态和湿态下的载荷强度分别为6.56和29.85N,植入肌肉及皮下后炎症反应较轻,有利于细胞贴附,并能促进角膜上皮的损伤修复。该材料基本符合组织工程角膜支架材料的要求,为组织工程角膜支架的研究指明了方向。

To study the feasibility of culturing corneal epithelial cells on chitin-based carrier and to investigate its promotion effect on corneal epithelial wound healing of New Zealand rabbits. The thickness, water content, transparency, mechanical strength, biocompatibility and degradation of the chitin membrane which was made by our laboratory were measured. To evaluate the cytocompatibility of the membranes with corneal epithelial cells cultured on the surface of the carrier membranes. Constructed the tissue engineering corneal epithelial, and then transplanted them to damaged corneal epithelia of New Zealand rabbits, the repair effect was ob- served. The thickness of CH was 0. 2mm. The water content of chitin-based membranes was 81. 59%. The transparencies on different wavelength were greater than 80%, the load intensity on dry state and wet state were 6.56 and 29.85N. Animal tissue compatibility experiments showed that no remarkable inflammation ap- peared. They were benefit to the cell-adhesion and promoted the corneal epithelial wound healing. There are significant differences between tested group and contrasted group. The material was basically in line with the requirements of cornea tissue engineering scaffold. It pointed out a direction for the research of cornea tissue en- gineering.