地塞米松预防新生大鼠缺氧缺血性脑病的研究

EFFECT OF DEXAMETHASONE ON NEONATAL HYPOXIC-ISCHEMIC ENCEPHALOPATHY

目的 研究 Dex在新生大鼠 HIE中的神经保护作用及机理。方法 :4日龄SD大鼠随机分为三组 ,分别给予生理盐水、不同剂量 Dex预处理 4天 ,然后缺氧处理。观察梗塞发生率、梗塞面积、非结扎侧皮层厚度和细胞密度 ,皮层细胞超微结构变化。结果 :Dex组梗塞发生率和面积低于对照组 ,大脑神经细胞的超微病理变化轻。Dex组大鼠体重、非结扎侧皮层厚度和细胞密度少于对照组 ,Dex组死亡率高于对照组。结论 :地塞米松预处理对新生大鼠 HIE具有保护作用 ,远期可抑制体格生长和大脑皮层发育。

To investigated the effect of dexamethasone on neonatal HIE. Using all 4-d-old Sprague-Dawley rat pups were randomized to three different treatment groups. Neonatal rats were treated with either saline or dexamethasone for 4 consecutive days. After ligation of the left common carotid artery, the rats were treated with hypoxia and decapitated on the 10 th ,14 th ,21 st and 28 th postnatal days. The area of infarction in the ligated side motor cortex and the density and thickness of cells in the non-ligated side cortex was measured, respectively. The supermicro-construction of neuronal cells in cortex was objected. Results showed; The area and rate of infarction, ligated side brain damage was lower in both Dex groups. The thickness and density of cells in cortical and weight of rats was lower in both Dex groups than that in the saline group. However, a high mortality rate was noted in the high dose Dex groups. Therefore, These findings suggest that the pretreatment with Dex protects the developing brain from HIE through the suppression of the neuronal maturation. We objected for a long time and fined rats body and cortex maturation were suppression in both Dex groups. It may give rise to psychomotor retardation later. (Original article on page 162)