广西130株非O1群霍乱弧菌血清学分型及噬菌体—生物分型研究

Study on serotyping and phage-biotyping of 130 V.cholerae non-O1 strains in Guangxi

目的将噬菌体—生物分型方法应用到非O1群霍乱弧菌的分型 ,以掌握广西地区非O1群霍乱弧菌血清型别的分布 ,并对此分型方法进行探讨。方法收集广西部分地区130株非O1群霍乱弧菌进行血清学分型和噬菌体—生物分型 ,并用多聚酶链反应 (PCR)进行霍乱肠毒素检测。结果在130株菌株中有90株可以分型 ,占69.23 % ,分布于34个血清型 ,腹泻病人菌株可分为14个血清型 ,其中以O2为优势血清型 ,外环境菌株分布于31个血清型 ,以O21、O32为优势血清型。107株非O1群霍乱弧菌共分为14个噬菌体型 ,4个生物型 ,用PCR方法检测其中17株非O1群霍乱弧菌肠毒素 (CT)为阴性。结论腹泻病人与外环境菌株的优势血清型别不同 ,而噬菌体—生物分型结果基本一致 ,肠毒素检测为阴性 ,因此 ,不具备引起流行的能力 ,为非流行株。

To understand the distribution of different serotypes of V.cholerae non-O1 in Guangxi and to evaluate the method of phage-bioty ping. Methods Serotyping and phage-biotyping systems were used to examine 130 V. cholerae non-O1 strains collected from some areas in Guangxi, and cholera toxin (CT) was detected by PCR.Results 90 strains (69.23%) were subdivided into 34 serotypes among 130 ones.The strains isolated from diarrhea patients were dividedinto 14 serotypes,with O2 predominant; the strains from environment were dividedinto 31 serotypes,with O1and O32 predominant.107 V.cholerae non-O1 strains were identified 14 phagetypes and 4 biotypes.CT detection of 17 strains by PCR was negative.Conclusion Predominant serotypes from diarrhea patients were different from those from environment,while the results of phage-biotyping were nearly the same .CT negative confirmed that these strains were non-epidemic ones and could not cause epidemic.