血管紧张素转化酶基因多态性与内皮功能的研究

The deletion/insertion polymorphism of the angiotensin converting enzyme gene and endothelial function

目的 :探讨血管紧张素转化酶 (ACE)基因多态性与冠心病血管内皮功能的关系。方法 :采用多聚酶链反应(PCR)技术对 2 4例心肌梗死、5 6例心绞痛患者、6 5例正常人的 ACE基因型及血浆内皮素 (ET)、内皮源性舒张因子 (EDRF)进行了检测 ,以 ET/ NO作为内皮功能指标。结果 :心肌梗死组 D等位基因频率显著高于正常对照组 ,DD型患者中的 ET/ NO比值较 型明显升高 (P<0 .0 5 )。结论 :DD型心肌梗死患者内皮功能受损较重。

Aim:to investigate the association between the deletion/insertion polymorphism of the angiotensin converting enzyme (ACE) gene and endothelial function in coronary heart disease. Methods:ACE genotype was determined by PCR,and plasma endothelin (ET 1),endothelium derived relaxing factor/nitric oxide (EDRF/NO) were meassured in 24 cases of myocardial infarction (MI),56 cases of angina and 65 normal subjects. Results:D allele frequency was increased,compared with normal control (52.1% vs 34.6%, P <0.05), and ET 1/NO ratio was higher in DD homozygotes than in II genotype (0.98±0.32 vs 0.63±0.22, P <0.05) in patients with MI. Conclusion:endothelial dysfunction may related to ACE DD genotype in patients of MI.