2型糖尿病患者胰升糖素受体基因的PCR-DGGE扫查

PCR-DGGE scanning of the glucagon receptor gene in Chinese patients with type 2 diabetes mellitus

目的 探讨胰升糖素受体 (GCG- R)基因在中国人 2型糖尿病遗传背景中的地位。方法 运用聚合酶链反应-变性梯度凝胶电泳 (PCR- DGGE)技术对 2 0 0例无亲缘关系之中国云南昆明地区汉族人 (2型糖尿病患者 12 0例 ,健康对照者 80例 )的 GCG- R基因编码区进行了分子扫查。结果 仅发现 2例 2型糖尿病患者及 1例健康对照者的 GCG- R基因第 7外显子存在多态变异带型 ,这一杂合子多态变异基因型在 2型糖尿病组和对照组中的发生频率分别为 0 .0 17和0 .0 13,两者之间无显著性差异。此外 ,研究对象 GCG- R基因其它编码区的 PCR- DGGE扫查均未发现异常泳动片段。结论 GCG- R基因可能不是中国昆明地区汉族人 2型糖尿病的主要致病基因。

Objective To probe the role of the glucagon receptor (GCG R) gene in the background of type 2 diabetes mellitus in Chinese.Methods A total of 200 unrelated Chinese subjects (120 patients with type 2 diabetes and 80 nondiabetic control individuals without family history of diabetes) from Kunming were screened for the presence of the polymorphic variants in codon domains of the GCG R gene by use of polymerase chain reaction denaturing gradient gel electrophoresis (PCR DGGE).Results Only a heterozygous polymorphic band was observed in exon 7 in 2 diabetics and 1 control person and no differences in the frequencies of this abnormal conformer were found between diabetic patients and control group.No other abnormal band patterns in codon regions of the GCG R gene were showed in all of the subjects investigated,including type 2 diabetics and controls.Conclusion The results suggested that the GCG R gene is not the causative gene responsible for the occurrence of type 2 diabetes mellitus in Chinese and indicated the genetic heterogeneity of type 2 diabetes mellitus in different ethnic populations.