反相高效液相色谱法测定双黄连口服液中黄芩甙含量

Determination of Baicaline in Shuanghuanglian Oral Liquid by RP HPLC

目的 :探讨双黄连口服液含量测定检测方法。方法 :利用反相高效液相色谱法 (RP-HPL C)测定双黄连口服液中黄芩甙含量。固定相为 Nova-Pak C1 8反相柱 ;流动相为甲醇 -水 -磷酸 (5 0∶ 5 0∶ 0 .2 ) ;检测波长为 2 76nm。结果 :该方法的线性范围为 0 .3 6～ 1 .79μg(r=0 .9996) ,平均回收率为 1 0 2 .62 % ,RSD=1 .5 6% (n=5 )。结论 :本法简便 ,准确 ,灵敏度高 ,重现性好 。

OBJECTIVE:The content of baicaline in Shuanghuanglian oral liquid was determined by RP HPLC.METHOD: RP HPLC analysis was carried out by using Nava Pak C 18 column and methanol water phosphoric(50∶50∶0.2) asthe mobile, the detection wave length was 276 nm.RESULTS: The method was linear withintherangeof0.36～1.79 μg(γ=0.999 6),the average recovery was 102.62%, the RSD=1.56%(n=5). CONCLUSION: The method was simple, accruate, highly sensitive and reproducible. It may be used for the quantitative determination of baicaline in Shuandhuanglian oral liquid.