甲型副伤寒沙门菌的抗生素耐药性及分子分型研究

Drug resistance and molecular subtyping of almonella paratyphi A by PFGE

目的了解2008年深圳市福田区腹泻病人中分离的甲型副伤寒沙门菌的耐药性及分子分型特征。方法从辖区内医院腹泻病人中分离得到11株甲型副伤寒沙门菌,选择10种抗生素,用K-B纸片扩散法进行药物敏感试验;细菌基因组DNA经限制性内切酶Xba I酶切,用脉冲场凝胶电泳进行分子分型,使用Quantity-One TM软件成像并使用BioNumerics 6.1软件对其相似性进行分析比较。结果11株甲型副伤寒沙门菌对氨苄西林、诺氟沙星、替卡西林等8种抗生素敏感率均为100%,对其余抗生素则出现中介或耐药菌株。对复方新诺明和氨苄西林敏感率为81.8%,四环素敏感率达90.9%。11株受试菌株的PFGE图谱经软件分析,可分为5个PFGE型,其相似性系数在93%以上。结论本次研究的甲型副伤寒沙门菌对大多数抗生素敏感;菌株间有较高的同源性。

Objective To survey the resistance to antibiotics and typing characteristics of Salmonella paratyphi A isolat- ed in diarrhea patient of Futian District, Shenzhen in 2008. Methods Eleven strains of Salmonella paratyphi A were isolated and identified from diarrhea patient of Futian District, and antimicrobial susceptibility test of the strains were conducted on by Kirby-Bauer methods using 10 kinds of antibiotics. Bacterial cells were lysised to release the bacteria genomic DNA, digested with restriction endonuclease Xba I. and then subjected to molecular subtyping by PFGE. Profiles were obtained with Quanti- ty - One TM software and their homological analysis was conducted by BioNumerics 6.1 software. Results Eleven strains of Salmonella paratyphi A were all susceptible to eight antibiotics, such as ticarcillin, amikacin, norfloxacin, piperacillin, et al. And intermediate or low resistance to other antibiotics. The susceptibility of the 11 strains to co-trimoxazole and anpicillin were 81.8% and 90.9%. Analysis of the PFGE profiles of the 11 strains of Salmonella paratyphi A indicated that all of the strains differentiated into 5 PFGE types, and similarity of 11 strains were above 93%. Conclusion Most of these Salmonella paratyphi A were susceptible to 10 antibiotics. These strains homology are relatively high.