苯对细胞增殖抑制率的影响及保护因素探讨

Effect of benzene on the inhibition rate of cell proliferation and investigation of relevant protective factors

目的探讨苯对骨髓单个核细胞增殖抑制率的影响,同时了解血清及氨磷汀能否作为保护因素减轻苯对细胞的影响。方法实验分四组:空白对照组、(0.25、3.5、20μmol/L)苯组、(0.25、3.5、20μmol/L)苯+(2、10、50μg/mL)氨磷汀组、(0.25、3.5、20μmol/L)苯+血清组,通过细胞增殖检测(CCK-8法)并计算骨髓单个核细胞增殖抑制率。结果高浓度苯组较低、中浓度苯组更能抑制细胞增殖(P<0.01);(3.5、0.25μmol/L)苯+血清组细胞增殖抑制率均较相对应浓度的苯组减少(P<0.05或P<0.01);但20μmol/L苯+血清组与苯组间细胞增殖抑制率差异无统计学意义(P>0.05)。(0.25、3.5、20μmol/L)苯+(50、10、2μg/ml)氨磷汀,细胞增殖抑制率与相应浓度苯组比较,结果均具有显著性差异(P<0.01或P<0.05)。结论细胞增殖抑制率随苯剂浓度的增加而增加,苯毒性存在剂量依赖效应;血清及氨磷汀均能减轻苯对细胞的增殖毒性,可能可以作为苯中毒细胞的保护剂。

Objective To explore the effect of benzene on the inhibition rate of proliferation of bone marrow mononudear cells and investigate the relevant protective factors. Methods The experiment included four groups： blank control group, （ 0.25, 3.5, 20μmol/L ） benzene group, （ 0.25, 3.5, 20μmol/L ） benzene ＋ （ 2, 10, 50μg/mL ） amifostine group, （ 0.25,3.5, 20μmol/L ） benzene＋serum group, the inhibition of proliferation of bone marrow mononuclear cell would be tested and calculat- ed by the cell proliferation assay （ CCK-8 method）. Results High concentrations of benzene could highly inhibit cell prolifer- ation than the low or medium concentrations groupa （P 〈 0.01 ）;The inhibition rate of cell proliferation were corresponding re- duced in （3.5, 0.25μmol/L）Benzene ＋ serum group than that in benzene group （P 〈0.05 or P 〈0.01 ）; but there was no signifi- cant difference between the 20μmollL Benzene ＋ serum group and the benzene group （P〉0.05）.Significant differences were no- ticed between（0.25, 3.5, 20μmol/L） benzene ＋（50, 10, 2ug/ml ）amifostine group and the benzene ones （P〈0.01 or P〈0.05 ）. Conclusion The inhibition rate of cell proliferation will increase with the increase of benzene concentration, and there is a dose-dependent effect in toxicity of benzene; The serum and amifostine may reduce the toxicity of benzene on cell proliferation and they perhaps act as a protectant for benzene poisoning cells.